Project 08

 

Consequences of chronic hypoxia and HIF-1/HIF-2 in regulating function and plastic-ity of macrophages and tumor cells



Principal Investigator:

Prof. Dr. Bernhard Br├╝ne
Institute of Biochemistry I
Goethe University Frankfurt / Main

Research Area:

Biochemistry, Inflammation, Pathobiochemistry

Summary:

The role of chronic hypoxia in affecting biological systems is rarely known, although affecting expression of genes via tristetraprolin. We determine the post-transcriptional regulation of genes via tristetraprolin using the individual-nucleotide resolution UV cross-linking and immunoprecipitation- and NGS-method, to understand the role of affected genes in macrophages. In addition, HIF-1 impairs the assembly of complex I of the mitochondrial respiratory chain by inducing ╬▓-TrCP (╬▓-transducin repeat-containing protein) and degrading the assembly factor TMEM126B. The relevance of this observation towards the functional role of mitochondria in determining macrophages and tumor cells will be evaluated in vitro and in vivo.

Links:

Institute of Biochemistry I (http://www.pathobiochemie1.de/)